diff --git a/lntest/itest/lnd_test.go b/lntest/itest/lnd_test.go
index 5817e85d..b7a4b1a6 100644
--- a/lntest/itest/lnd_test.go
+++ b/lntest/itest/lnd_test.go
@@ -14761,6 +14761,10 @@ var testsCases = []*testCase{
 		name: "cpfp",
 		test: testCPFP,
 	},
+	{
+		name: "macaroon authentication",
+		test: testMacaroonAuthentication,
+	},
 }
 
 // TestLightningNetworkDaemon performs a series of integration tests amongst a
diff --git a/lntest/itest/macaroons.go b/lntest/itest/macaroons.go
new file mode 100644
index 00000000..bfade118
--- /dev/null
+++ b/lntest/itest/macaroons.go
@@ -0,0 +1,168 @@
+// +build rpctest
+
+package itest
+
+import (
+	"context"
+	"strings"
+
+	"github.com/lightningnetwork/lnd/lnrpc"
+	"github.com/lightningnetwork/lnd/lntest"
+	"github.com/lightningnetwork/lnd/macaroons"
+	"gopkg.in/macaroon.v2"
+)
+
+// errContains is a helper function that returns true if a string is contained
+// in the message of an error.
+func errContains(err error, str string) bool {
+	return strings.Contains(err.Error(), str)
+}
+
+// testMacaroonAuthentication makes sure that if macaroon authentication is
+// enabled on the gRPC interface, no requests with missing or invalid
+// macaroons are allowed. Further, the specific access rights (read/write,
+// entity based) and first-party caveats are tested as well.
+func testMacaroonAuthentication(net *lntest.NetworkHarness, t *harnessTest) {
+	var (
+		ctxb       = context.Background()
+		infoReq    = &lnrpc.GetInfoRequest{}
+		newAddrReq = &lnrpc.NewAddressRequest{
+			Type: AddrTypeWitnessPubkeyHash,
+		}
+		testNode = net.Alice
+	)
+
+	// First test: Make sure we get an error if we use no macaroons but try
+	// to connect to a node that has macaroon authentication enabled.
+	conn, err := testNode.ConnectRPC(false)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel := context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	noMacConnection := lnrpc.NewLightningClient(conn)
+	_, err = noMacConnection.GetInfo(ctxt, infoReq)
+	if err == nil || !errContains(err, "expected 1 macaroon") {
+		t.Fatalf("expected to get an error when connecting without " +
+			"macaroons")
+	}
+
+	// Second test: Ensure that an invalid macaroon also triggers an error.
+	invalidMac, _ := macaroon.New(
+		[]byte("dummy_root_key"), []byte("0"), "itest",
+		macaroon.LatestVersion,
+	)
+	conn, err = testNode.ConnectRPCWithMacaroon(invalidMac)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel = context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	invalidMacConnection := lnrpc.NewLightningClient(conn)
+	_, err = invalidMacConnection.GetInfo(ctxt, infoReq)
+	if err == nil || !errContains(err, "cannot get macaroon") {
+		t.Fatalf("expected to get an error when connecting with an " +
+			"invalid macaroon")
+	}
+
+	// Third test: Try to access a write method with read-only macaroon.
+	readonlyMac, err := testNode.ReadMacaroon(
+		testNode.ReadMacPath(), defaultTimeout,
+	)
+	if err != nil {
+		t.Fatalf("unable to read readonly.macaroon from node: %v", err)
+	}
+	conn, err = testNode.ConnectRPCWithMacaroon(readonlyMac)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel = context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	readonlyMacConnection := lnrpc.NewLightningClient(conn)
+	_, err = readonlyMacConnection.NewAddress(ctxt, newAddrReq)
+	if err == nil || !errContains(err, "permission denied") {
+		t.Fatalf("expected to get an error when connecting to " +
+			"write method with read-only macaroon")
+	}
+
+	// Fourth test: Check first-party caveat with timeout that expired
+	// 30 seconds ago.
+	timeoutMac, err := macaroons.AddConstraints(
+		readonlyMac, macaroons.TimeoutConstraint(-30),
+	)
+	if err != nil {
+		t.Fatalf("unable to add constraint to readonly macaroon: %v",
+			err)
+	}
+	conn, err = testNode.ConnectRPCWithMacaroon(timeoutMac)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel = context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	timeoutMacConnection := lnrpc.NewLightningClient(conn)
+	_, err = timeoutMacConnection.GetInfo(ctxt, infoReq)
+	if err == nil || !errContains(err, "macaroon has expired") {
+		t.Fatalf("expected to get an error when connecting with an " +
+			"invalid macaroon")
+	}
+
+	// Fifth test: Check first-party caveat with invalid IP address.
+	invalidIpAddrMac, err := macaroons.AddConstraints(
+		readonlyMac, macaroons.IPLockConstraint("1.1.1.1"),
+	)
+	if err != nil {
+		t.Fatalf("unable to add constraint to readonly macaroon: %v",
+			err)
+	}
+	conn, err = testNode.ConnectRPCWithMacaroon(invalidIpAddrMac)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel = context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	invalidIpAddrMacConnection := lnrpc.NewLightningClient(conn)
+	_, err = invalidIpAddrMacConnection.GetInfo(ctxt, infoReq)
+	if err == nil || !errContains(err, "different IP address") {
+		t.Fatalf("expected to get an error when connecting with an " +
+			"invalid macaroon")
+	}
+
+	// Sixth test: Make sure that if we do everything correct and send
+	// the admin macaroon with first-party caveats that we can satisfy,
+	// we get a correct answer.
+	adminMac, err := testNode.ReadMacaroon(
+		testNode.AdminMacPath(), defaultTimeout,
+	)
+	if err != nil {
+		t.Fatalf("unable to read admin.macaroon from node: %v", err)
+	}
+	adminMac, err = macaroons.AddConstraints(
+		adminMac, macaroons.TimeoutConstraint(30),
+		macaroons.IPLockConstraint("127.0.0.1"),
+	)
+	if err != nil {
+		t.Fatalf("unable to add constraints to admin macaroon: %v", err)
+	}
+	conn, err = testNode.ConnectRPCWithMacaroon(adminMac)
+	if err != nil {
+		t.Fatalf("unable to connect to alice: %v", err)
+	}
+	defer conn.Close()
+	ctxt, cancel = context.WithTimeout(ctxb, defaultTimeout)
+	defer cancel()
+	adminMacConnection := lnrpc.NewLightningClient(conn)
+	res, err := adminMacConnection.NewAddress(ctxt, newAddrReq)
+	if err != nil {
+		t.Fatalf("unable to get new address with valid macaroon: %v",
+			err)
+	}
+	if !strings.HasPrefix(res.Address, "bcrt1") {
+		t.Fatalf("returned address was not a regtest address")
+	}
+}